opioid receptor mu 1 gene  (OriGene)

Journal: Cell reports

Article Title: Dopamine D2 receptors bidirectionally regulate striatal enkephalin expression: Implications for cocaine reward

doi: 10.1016/j.celrep.2022.111440

Figure Lengend Snippet: Expression of specific genes is quantified with qPCR and gene chip microarray for mice overexpressing D2Rs (A–D) or following selective loss of D2Rs from D2-MSNs (E and F). (A) Schematic shows Drd2 mRNA levels are increased over wild type (pink) in mice expressing the human Drd2 transgene (D2R-OE) and in the absence of doxycycline-supplemented chow (dark red). (B) Striatal preproenkephalin ( Penk ) mRNA levels in D2R-OE mice (n = 4–5) or controls (n = 5). When Drd2 transgene expression was suppressed (ON Dox, pink) Penk mRNA was similar to controls. Turning on Drd2 transgene (OFF Dox, dark red) reduced Penk mRNA (B) and cRNA (C) compared with controls (t test). (D) Correlation of Penk levels between gene chip microarray and qPCR when the transgene is expressed (OFF Dox, red) in D2R-OE and control mice. (E) Schematic shows decreased Drd2 mRNA levels in mice with a homozygous deletion of Drd2 from D2-MSNs (MSN- Drd2 KO, white) relative to wild type (gray). (F) MSN- Drd2 KO mice (n = 3) show increased Penk mRNA, but no change in dynorphin ( Pdyn ) or δ- and μ -opioid receptors ( Oprd1 , Oprm1 ) compared with littermate controls (gray, n = 4; t test). (G–I) Ten days of non-contingent (G; 20 mg/kg, intraperitoneally) or contingent (H; mean: 17 ± 3 mg/kg, intravenous self-administration) cocaine followed by 14 days of withdrawal increased striatal Penk mRNA in C57Bl/6J. (I) Five days of non-contingent (15 mg/kg, intraperitoneally) cocaine followed by 14 days of withdrawal reduced Penk mRNA in C57Bl/6J, but not MSN- Drd2 KO mice (J). ns = not significant;*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Mean ± SEM and/or individual values shown.

Article Snippet: TaqMan Gene Expression Assays: mu 1 opioid receptor , Thermo Fisher , Mm01188089_m1.

Techniques: Expressing, Microarray, Control

Journal: Cell reports

Article Title: Dopamine D2 receptors bidirectionally regulate striatal enkephalin expression: Implications for cocaine reward

doi: 10.1016/j.celrep.2022.111440

Figure Lengend Snippet: (A–C) Top, Representative oIPSC traces recorded from MSNs in Adora2a-Cre or MSN- Drd2 KO in presence of the μ-opioid receptor agonist DAMGO (1 μM, orange), enkephalin (1 μM) + peptidase inhibitors (2 μM thiorphan + 10 μM bestatin; blue), or the opioid receptor antagonist naloxone (5 μM; red). Bottom, Time course of oIPSC amplitude expressed as percent of baseline recorded from ChR2-negative MSNs from Adora2a-Cre (n = 5 cells/2 mice) and MSN- Drd2 KO mice (C, n = 10 cells/3 mice). (D) Representative spontaneous IPSC (sIPSC) traces recorded from MSNs in Adora2a-Cre (left) or MSN- Drd2 KO (right) are shown in the presence of enkephalin + peptidase inhibitors (blue) or naloxone (red). (E and F) Number of sIPSCs plotted by amplitude for Adora2a-Cre (left, n = 4 cells/2 mice) or MSN- Drd2 KO (right, n = 8 cells/3 mice) during enkephalin + peptidase inhibitors (E) or naloxone (F) relative to peptidase inhibitor baseline (black). (G) sIPSC frequency was enhanced by naloxone in both genotypes. Parametric and non-parametric tests performed (see text). #p = 0.05–0.07, *p < 0.05, **p < 0.01, ****p < 0.0001. Mean ± SEM and/or individual values/traces shown.

Article Snippet: TaqMan Gene Expression Assays: mu 1 opioid receptor , Thermo Fisher , Mm01188089_m1.

Techniques:

Journal: Cell reports

Article Title: Dopamine D2 receptors bidirectionally regulate striatal enkephalin expression: Implications for cocaine reward

doi: 10.1016/j.celrep.2022.111440

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: TaqMan Gene Expression Assays: mu 1 opioid receptor , Thermo Fisher , Mm01188089_m1.

Techniques: Virus, Plasmid Preparation, Recombinant, Saline, Reverse Transcription, Gene Expression, SYBR Green Assay, Software